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Cells at the exponential period ended up spread on a MY plate and even further incubated at 30oC for four to six h in advance of harvesting for microscopy. Cells were mounted with a resolution of three% paraformaldehyde in a 50mM-phosphate buffer that contains 1mM-MgCl2 (pH 6. Just after washing with the buffer, cells were addressed with Novozyme 234 (Novo Industri A/S, Bagsvaerd, Denmark) for sixty min at 30oC with reciprocal shaking to clear away the mobile wall. […]

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